Plant mannan oligosaccharides
Healthy gut,
Adjust? immunity,
Leading the future.
Technological innovation.
河北吉農久遠生物科技有限公司
Hebei Jinong Jiuyuan Biotechnology Co., Ltd
Jinong Jiuyuan
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INTRODUCTION
Mammals themselves are not sufficient to maintain health and require microbial metabolites to prevent diseases. The vast majority of microorganisms in mammals are concentrated in the digestive tract, which is a complex ecosystem and a diverse and highly evolved microbial community. Different bacteria in the intestine have their preferred feeding patterns.
For example, long bifidobacteria isolated from the gut of young mammals prefer short chain? Human milk oligosaccharides （HMOs） over complex high molecular weight HMOs; Different bifidobacteria have different strategies for utilizing oligosaccharides. Bifidobacterium longum and Bifidobacterium infantis compete for the uptake of HMOs. Bifidobacterium longum excels in consuming HMOs through oligosaccharide binding proteins, while Bifidobacterium infantis uses several sets of glycolytic enzymes to first degrade complex HMOs before ingesting them into the cell.
? ? ? ? ? ? ? ? ? ? ?(Image above: Bifidobacterium)
Unlike probiotics, the oligosaccharide binding proteins of Salmonella enteritidis are more inclined to bind to monosaccharides β- D-glucose β- D-galactose. Exploring the dietary profile of probiotics is beneficial for producing high-quality prebiotics.
(Left image: Salmonella)
Composed of 2-10 mannose and glucose or galactose residues, passed through α- 1,2 、α- 1,3 、α- 1,6 、β- 1,4 β- 1,6 Oligosaccharides composed? glycosidic chain connections.
Main sources:? sesbania , coffee beans, palm fruits, guar beans, etc.
Mannose oligosaccharides ≥ 32%, galactomannan oligosaccharides ≥ 12%, glucuronic acid oligosaccharides ≥ 1.5%, arabinose xylobiose and xylotriose ≥ 2%.
Sweetness lower than sucrose; The viscosity will decrease with increasing temperature and then rise again after cooling; When the pH value is 1.5-3, the viscosity rapidly increases, and when the pH value is 3-9, the viscosity stabilizes; When the pH value is between 2.5-9, it is quite stable and almost does not decompose at a temperature of 100 ℃ for one hour.
Characteristics of Plant Mannose Oligosaccharides：
1. A specific L-chain fucosyllactose residue, closest to HMOs, effectively establishes an endogenous dominant gut microbiota, and promotes significant proliferation of immunoglobulins;
2. Must have three criteria: resistance to gastric acid, host enzyme hydrolysis, and gastrointestinal absorption;
3. Only 1% is absorbed, 1-2% is excreted in the form of feces and urine, and the vast majority can be selectively utilized by microorganisms in the distal small intestine and colon.
CONCEPT
COMPOSITION
HMOs - the theoretically optimal and rational oligosaccharide structure
PHYSICAL
PROPERTY
Plant derived mannan oligosaccharides
Main glycosidic chains：α-1,2、 α-1,3 、α-1,6、 β-1,4、β-1,6 , etc；
Side chains: D-mannose, L-fucosyllactose residues.
Technological Innovation - Leading the Future
Plant mannan oligosaccharides are a new type of oligosaccharide developed by our team through studying the preferences of different gut microbiota, searching for oligosaccharide binding proteins of probiotics, characterizing probiotic feeding patterns, and better regulating gut microbiota with precision. Using oligosaccharide binding proteins as probes, this new oligosaccharide mainly composed of mannan oligosaccharides is more conducive to precise regulation of gut microbiota and promoting body health.
1. There is a protein BIMnBP1 in Bifidobacterium that can bind to mannose oligosaccharides,? BIMnBP1 can interact with mannose disaccharides, mannose trisaccharides, mannose pentasaccharides, etc. The interface between BIMnBP1 and mannan oligosaccharides includes both direct hydrogen bonding interactions and indirect hydrogen bonding interactions mediated by water molecules.
FUNCTION MECHANISM
A is the interaction between protein BIMnBP1 and mannose disaccharides, mannose trisaccharides, and mannose pentasaccharides;
B is the detailed action of protein BIMnBP1 through positively charged amino acids K126, K152, and K287 through mannan oligosaccharides. (Figure 1)
A is the protein BiF12BP that interacts with lactose-N-disaccharide through six residues;
B is the protein BiF12BP that interacts with galacto-N-diose through 5 residues;
C is the protein BiF12BP that interacts with lactose-N-tetrasaccharides through 8 residues. (Figure 2)
A is the details of the interaction between protein BIAXBP and arabinobiose;
B is the protein BIAXBP that interacts with arabinobiose and arabic xylotriose through polar amino acids N72, N39, and D386, respectively. (Figure 3)
Mannose oligosaccharides have different sugar chain lengths, and low degree oligosaccharides are present in the empty pockets of BIMnBP1. For example, mannose pentasaccharides can form hydrogen bonds with the 16 amino acids of BIMnBP1, while mannose trisaccharides and mannose disaccharides can form hydrogen bonds with the 10 and 6 amino acids of BIMnBP1, respectively. The glycosidic chain of mannan oligosaccharides can form the most direct salt bridge interaction with BIMnBP1 positively charged amino acids.
2. There is a protein BiF12BP in Bifidobacterium that can bind to fucosylactose, which can recognize 2 '- fucosylactose and 3-fucosylactose. In addition to hydrogen bonding, fucosylactose also forms hydrophobic interactions with a series of non-polar amino acids such as N39, A88, and F92 through van der Waals forces during its interaction with BiF12BP. This hydrophobic interaction greatly enhances the binding ability of BiF12BP to substrates. Fucosylactose is an important component of HMOs, which can be ingested by Bifidobacterium longum and produce lactic acid and fatty acids, significantly reducing intestinal pH.
However, pathogenic bacteria in the intestine, such as Campylobacter jejuni, Enteropathogenic Escherichia coli, and Salmonella enteritidis, cannot utilize fucosylactose and their growth is inhibited (possibly due to low pH environments). Fucosylactose can directly downregulate the expression of CD14, affecting the recognition and binding of lipopolysaccharides in Gram negative bacteria, thereby reducing the colonization of Gram negative bacteria in the intestine. Feeding lactating young animals with feed containing fucosylactose can achieve similar levels of breastfeeding with lower levels of inflammatory cytokines. Lactose-N-disaccharides, galacto-N-diccharides, and lacto-N-tetrasaccharides are important components of HMOs and play important roles in the normal development of young animals. They can specifically promote the proliferation of bifidobacteria in the intestinal tract of young animals.
3. There is a protein BIAXBP in Bifidobacterium that can bind to arabinoxylo-oligosaccharide. The interface between BIAXBP and its substrate mainly consists of hydrogen bonding interactions and water molecule mediated hydrogen bonding interactions. In the process of BIAXBP participating in the recognition of arabinoxiose, a total of 10 amino acids participate in direct hydrogen bonding interactions with substrates,
a total of 10 amino acids participate in direct hydrogen bonding interactions with substrates, they are N39, S41, A42, N72, E73, F75, A76, L96, LQ254, and D386, respectively. Arabinoxigosaccharides has the ability to promote the growth of human colon bifidobacteria and produce butyrate.
4. The plant lectins on the surface of pathogens have specificity for mannose and can specifically bind to receptors of glycoprotein like oligosaccharide structures in intestinal epithelial cells. When this binding cannot proceed normally, the pathogen will not have a pathogenic effect on the host. Simultaneously reduce the inactivation effect of bile acids, hydrochloric acid, etc. on probiotics. The addition of non fermented plant derived mannan oligosaccharides from external sources can interfere with the recognition and adhesion ability of pathogens to intestinal epithelial cells, forming a barrier between animal intestinal epithelial cells and pathogens.
The Structure of Oligosaccharide Binding Proteins
（Figure 2）
(Figure 1)Spatial structural information of mannan oligosaccharide binding protein in Bifidobacterium
(Figure 3)Spatial structural information of arabinoxylo-oligosaccharide binding protein in Bifidobacterium
（180°）
1. Adsorption of pathogenic bacteria and fungal toxins (galactomannan oligosaccharides are the main components of microbial cell walls): The function of galactomannan oligosaccharides is similar to the floating bridge of life. Pathogens adhere to oligosaccharide substrates without contact with animal intestinal epithelial cells, and cannot settle in the intestine, thus losing their harm to the body.
MAIN EFFECT
3. Healthy gut, reducing the generation of harmful substances, repairing intestinal damage, and promoting intestinal villus growth: Mannan oligosaccharides are an important semi cellulose polymer that can be utilized by intestinal microorganisms such as Bifidobacterium adolescens, Lactobacillus gaili, and Lactobacillus, but not by harmful bacteria such as Clostridium perfringens, Escherichia coli, and Salmonella that produce harmful substances.In addition to the common physiological effects of other functional oligosaccharides, it also has the function of clearing free radicals and enhancing the body's antioxidant capacity. Mannose oligosaccharides can proliferate a large number of beneficial bacteria such as bifidobacteria. As a filler, beneficial bacteria occupy a space in the intestine, preventing the colonization of conditional pathogenic bacteria and exogenous bacteria, and preventing the occurrence of intestinal inflammation; The secondary metabolites of beneficial bacteria, such as short chain fatty acids, can stimulate epithelial cell turnover and reduce gastrointestinal pH, compressing bacteria that are intolerant to high acidity. After being fermented by the gut microbiota, mannan oligosaccharides ultimately produce short chain fatty acids such as acetic acid, propionic acid, and n-butanol, which is beneficial for regulating intestinal health.
2. Increase immunoglobulin levels; Enhance phagocytic cells and macrophages; Eliminate immune suppression and reduce liver burden; Reduce the generation and absorption of intestinal ammonia, and alleviate the liver's detoxification burden on blood ammonia; Reduce the occurrence of allergies and inflammatory enteritis. Mannose oligosaccharides can affect the immune system by stimulating the liver to secrete mannose oligosaccharide binding proteins.
Target audience and Usage
- 200g~1000g/ton for? remove mycotoxin and detoxification;
- 200g~ 500g /ton for long term health care ;
- Piglets and sows 500g~2000g/ton, growing and fattening pigs 200g~1000g/ton, poultry 300g~500g/ton, aquatic animals 1000g~3000g/ton, can double useage in cases of weakened immunity and toxin poisoning.
Packaging: 25kg/bag;?
Storage: Sealed and stored in a ventilated, cool, and dry place.
Shelf life: 24 months.
Healthy gut, Protecting Immunity
5. Plant diversity oligosaccharides can promote the growth of bifidobacteria while inhibiting the excretion of cresol by urinary toxins (promoting the conversion of nitrogen from urethral excretion to fecal excretion, which is a beneficial conversion and reduces kidney damage). When Bifidobacterium longum and Eubacterium rectale are co cultured, Bifidobacterium longum consume the arabinose of AXOS and produce acetate, which is then fermented by Eubacterium rectale to produce butyrate.
CONTACT US
Jinong Jiuyuan
Manufacturer: Hebei Jinong Jiuyuan Biotechnology Co., Ltd
Production/Registration Address: North Industrial Park, Wangweitun Village, Machang Town, Qing County, Cangzhou City, Hebei Province, China
Phone: 0317-4196677 Postal Code: 062650
TECHNOLOGICAL INNOVATION
LEADING THE FUTURE
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